![]() However, when it comes to WSI, not the format but the size of the image is the problem. ImageJ is compatible with over 150 different biological image formats called the Bio-Formats, WSI included. Many of them have a simple graphical user interface (GUI), but some require programming skills to use. Over the years a large set of ImageJ plugins, covering most areas of biological image analysis has been developed. For use in a regulated environment, a separate validation and verification would be required by the end user. Even though the program is so widely used, ImageJ is an experimental system and NIH does not assume any responsibility for its use by other parties. ImageJ is probably the best known and longest-lived open source software for biomedical image analysis. ![]() ImageJ is a Java-based image processing program developed as a collaboration between the National Institutes of Health ( NIH) and Laboratory for Optical and Computational Instrumentation ( LOCI) at the University of Wisconsin.
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